The Antioxidant Activity of Litsea oppositifolia Stem Extract Against Oxidative Models on 3T3 Fibroblast Cell Line

Abstract

Skin aging is the accumulation of cellular waste that progressively leads to a loss of structural integrity and physiological function due to intrinsic and extrinsic factors. Although intrinsic aging is inevitable, it is highly influenced by extrinsic factors, including UV radiation, tobacco smoking and pollution. These repeated exposure causes the upper dermis fibroblasts to selectively lose through oxidative damage caused by ROS which gradually reduces the production of the ECM. A natural compound found in Litsea oppositifolia stem extract was shown to exert antioxidant activity that can eliminate ROS. Hence, this study was done to evaluate the antioxidant activity of Litsea oppositifolia stem extract using 3T3 fibroblast cell line treated with H2O Litsea oppositifolia stem extract was obtained from the Pharmacy Department of University of Indonesia in the form of paste. Various concentrations of the extract, ascorbic acid, and HO22 were prepared and treated to the cells to evaluate the cytotoxicity effects. The results show that Litsea oppositifolia stem extract was less toxic compared to ascorbic acid. The antioxidant assay was done afterwards using 50 μM H induced oxidative stress and the extract showed that the concentration of 6.25 ppm displayed the most optimal protection against H2O. This suggests that at a concentration of 6.25 ppm, Litsea oppositifolia stem extract would serve as the ideal concentration to be produced as cosmetics.