Histological Evaluation of Wound Healing Treatment with Pectin clindamycin and Keratin-Pectin clindamycin

Abstract

Keratin, pectin, hydrogel, and clindamycin are the materials that exert a good effect to promote a better wound healing process. Each material exerts a specific effect that can support the wound healing process. Keratin and pectin alone have poor mechanical and intrinsic factors which will need to be combined to exert their best potential for wound treatment. Clindamycin addition will promote better wound healing process. This study aims to investigate the pectin incorporated with clindamycin and keratin-pectin incorporated with clindamycin that is packed into hydrogel effectivity for wound treatment histologically. The mice were treated with pectin clindamycin and keratin pectin clindamycin wound dressing, measuring wound size reduction at allocated times. In addition, the skin tissue was extracted, sectioned, stained with hematoxylin and eosin, and observed under the microscope. On day 9, the pectin clindamycin treatment had significant results compared to the control (P < 0.0001). The keratin-pectin clindamycin treatment has significant results compared to the control (P < 0.0001). The statistical analysis results also show that this pectin clindamycin and keratin-pectin treatment on day 9 has no significant difference (P = 0.9521).This study shows that pectin clindamycin and keratin-pectin clindamycin treatment has a significant difference on wound size reduction (P < 0.0001) compared to the control group on the last day of the observation, which is on day 9. In histological evaluation, the pectin clindamycin and keratin-pectin clindamycin treatment can promote faster re-epithelialization, which means promoting a faster wound healing process than the control group. The pectin clindamycin and keratin-pectin treatment on day 9 have no significant difference (P = 0.9521).