CELL CULTURING AND PREPARATION IN BIOMANUFACTURING HANDBOOK V1.0

Abstract

Biomanufacturing industries are one of the steadily growing industries all over the world and predicted to be growing steadily and consistently over next decades. The needs of healthcare and its associated products and services are expected to rise proportionately with the growth of global human population. Biomanufacturing-based products are among the most highly cost-, labor-, skill- and knowledge- in healthcare products. This requires extensive training for the personnel to gain sufficient competency to operate. This handbook marks the upstream part of Biomanufacturing, covering the initiation of making cell culture stock and increase it up to the production levels, until the cell banking stage. The model cell culture described in this handbook is Chinese Hamster Ovary (CHO) cells that are commonly used as mammalian cell-culture based system and production. To enhance the proficiency of the personnel in cell culturing. Practise and guidelines in laboratory setting is also provided in this handbook. Basic aseptic techniques should be properly mastered before as one of the prerequisites to effectively acquire the learning outcome delivered in this handbook. This handbook is designed for Community Service program served to establish, improve and enhance biomanufacturing process with our partner company, PT Kalbio Global Medika. Implementation and development of methods described in this handbook is possible in many other related cell-culture based application in biomanufacturing settings. The proficiency level required to fully understand this book is intended for user(s) who is/are familiar with basic biological and chemical science and technology.