Investigation of The Effectiveness of P-compound Towards The Inhibition of Influenza Viral Replication

Abstract

Influenza virus can cause various symptoms in the human body ranging from mild to severe effects, leading to severe complications in some individuals. This virus replicates very quickly inside the cells in the human body. The existence of vaccines and drugs only focus on preventing the virus by inhibiting the neuraminidase of the Influenza virus and only targeting the body's immune system to help recognize the virus inside the human body still causes side effects; there is a chance the virus can still inject and replicate inside the human body. In addition, P-compound are known have the ability to directly target host cells by preventing the endosome from undergoing acidification. However, the deeper understanding of the mechanism and specific condition of concentration that could be used for influenza virus infection research development remains unclear. Therefore, through this study, the validation of finding of the effectiveness of the P-compound towards the inhibition of replication IAV was conducted by also further finding suitable concentrations of 12.5 μM and 12 hours incubation time conditions of this drug can be used for future investigation to inhibit the influenza virus replication validated by conducting Influenza virus infection assay, Plaque assay, Western blot, and RT-qPCR to examine the gene expression of the infected cells after treatment.