In vitro Investigation on The Anti-adherence and Antibacterial Effect of Lactic Acid Bacteria Isolated from Cincalok on Listeria monocytogenes"

Abstract

Humans have been co-existing with a whole population of microbes, where trillions of different microbes are living inside the human GI tracts as a commensal gut microbiota. This condition provides a mutual relationship where the gut microbiota serves as a natural protection for the intestinal tract homeostasis. Nevertheless, the human intestinal tract is constantly exposed to environmental microorganisms through foods, which greatly affects the gut integrity. Probiotics, such as LAB, that are commonly contained in cincalok provide benefits for food preservation and intestinal health. On the other hand, estimation of 600 million cases of foodborne diseases around the world have been reported, where notable pathogens, such as Listeria monocytogenes, are responsible for the increasing cases of foodborne illnesses due to unsafe foods. Considering the extensive benefits of LAB, this research investigated the microbe-host interactions, antibacterial and anti-adherence properties of LAB isolated from cincalok against Listeria monocytogenes in HT29 cells through an in vitro study of gene analysis using qRT-PCR, broth microdilution and anti-adherence assay. The study found that LAB isolated from cincalok possessed an anti-adherence property against L. monocytogenes. However, due to a number of limitations, it is suggested that future research is implemented to further understand the mechanisms of antimicrobial properties of LAB and explore the effect of different MOIs with identified LAB isolates.