Elucidating The Impact of EBV Infection on Cell Cycle Dynamic

Abstract

This research aims to analyze the impact of EBV (Epstein-Barr virus) infection on cell cycle phases (G1, S, G2/M) during latent and lytic phases, exploring alterations in cell cycle regulatory proteins. The investigation extends to compare fixation methods' ethanol and paraformaldehyde. The focus lies in comprehending EBV's effect on host cell cycle progression, emphasizing viral reactivation. The study employs D98 cell lines, selectively targeting EBV gene-containing cells with puromycin. Tamoxifen stimulation prompts viral reactivation after cell synchronization with RO-3306. Cell cycle analysis, achieved through propidium iodide (PI) staining using flow cytometry. Expected outcomes anticipate distinctive cell cycle distributions between latent and lytic phases, especially within G1, S, and G2/M phases. Additionally, the hypothesis suggests ethanol fixation may yield superior results, considering its ability to penetrate cells. This research broadens understanding regarding EBV's impact on host cell cycle regulation during different infection phases. It has implications for elucidating viral replication mechanisms and potential therapeutic targets