Evaluating the Role of Gene X in Glioblastoma Tumorigenesis and Temozolomide Resistance In Vitro

Abstract

Temozolomide (TMZ) stands as the primary chemotherapy choice for glioblastoma multiforme (GBM), yet resistance frequently emerges in patients, leading to tumor recurrence and worsened prognoses. A recent study reported upregulation of glutamine and glutamate transport and metabolism in TMZresistant GBM in silico, particularly highlighting the upregulation of Gene X. Given the reported glutamine addiction in gliomas, the current study aims to investigate the role of Gene X in the tumorigenesis and TMZ resistance in GBM by inducing Gene X knockdown in TMZ-sensitive Pt#3 and TMZ-resistant Pt#3-R GBM cell lines through shRNA-mediated knockdown by lentivirus transduction. The effects of Gene X knockdown were evaluated towards cell proliferation and migration. While repetition of the experiment remains necessary due to anomalous data of one transduction batch and inconclusive knockdown validation at a protein level, the current findings still suggested the role of Gene X in mediating TMZ resistance of Pt#3-R cells by affecting their proliferation and migration capabilities.