Biochemical Assay in Mus musculus as Subchronic Toxicity Study Using Foeniculum Vulgare Extract

Abstract

Foeniculum vulgare or known as adas in Indonesia is a medicinal plant that belongs to the umbelliferae family. It has a lot of pharmacological properties such as antiviral, antimicrobial, antioxidant, anti-inflammatory, anti-anxiety, gastro-protective, estrogenic-like, cardiovascular, lipid-lowering, anti-mutagenic, anti-diabetic, anti-cancer activity, hepatoprotective, and memory-protective characteristics. Hence, adas has been suggested as a potential therapy. Toxicity studies of adas extract were poorly investigated. The current research used Mus musculus mice as the animal model and investigated the subchronic toxicity of the adas extract using a biochemical assay to ensure its quality and safety as a potential treatment. The study was carried out in accordance with the Organisation for Economic Co-operation and Development (OECD) regulations. The findings were then utilized in statistical analysis (unpaired t-test) to produce the more valid and reliable result. However, some of the data collected less than three data which was not recommended to use unpaired t-test. The biochemical test also showed a negative value which was caused by technical and human error during the experiment. The present study used doses of 100 mg/kg body weight (BW) adas extract that was given orally to each mice. The toxicity assessment was done by calculating the level of each biochemical parameter to determine any adverse effects from adas extract towards vital organs.