Key Challenges in Biosimilar Development: Insights from the Indonesian Biopharmaceutical Sector See

Biosimilars are crucial therapies for chronic and life-threatening diseases. Indonesia presents a substantial market for biosimilars, with a large population of 280 million and the largest economy in Southeast Asia. Nevertheless, biosimilar development in Indonesia still encounters various obstacles. This study aims to identify and analyze key challenges in biosimilar development in Indonesia and provide strategic insights for industry stakeholders and regulators. This study employed a qu...

Development and Reformulation of Anti-platelet Drugs and Health Supplements Tablets in PT. Kalbe Farma See

This report outlines the pharmaceutical formulation internship conducted at PT Kalbe Farma Cikarang, emphasizing hands-on laboratory work, scale-up observation, and regulatory-driven reformulation projects. The internship centered on two key activities: replacing titanium dioxide in an antiplatelet drug coating and eliminating erythrosine from a health supplement coating. Laboratory tasks included granule testing, tablet performance evaluations, and monitoring pilot-scale manufacturing, ...

Optimization of Post-Processing Treatments of Honey as a Basis for Developing Functional Honey-Based Products with Enhanced Stability See

Post-processing treatments for Multiflora and Randu honey to improve the stability of honey-based functional products. The research evaluated the effects of thermal processing (40°C and 70°C) and acidification (citric acid) on Diastase Number (DN), Hydroxymethylfurfural (HMF), and microbial safety over 2 months. The results identified the 40°C Production treatment (30 minutes) as the optimal industrial standard, maintaining Diastase activity from 33.72 from 0 months to 13.05 in 2 months....

Quantitative Bright-Field Morphological Profiling as a Framework in Evaluating Cell Identity of Human Adipose-Derived Stem Cells (hADSC) See

Human adipose-derived stem cells (hADSCs) are widely used in regenerative medicine, yet verifying their undifferentiated morphology typically relies on molecular assays that may not always be accessible. This study aimed to establish a bioinformatic-related quantitative, image-based method to distinguish hADSCs from chondrocytes and to evaluate whether an experimental hADSC population maintains its expected stem cell morphology. Bright-field images of experimental hADSCs, control hADSCs,...

Investigating Novel Synta66-Derived ORAI1 Inhibitors For Cytotoxic Activity Against Glioblastoma See

Glioblastoma multiforme (GBM) is the most lethal primary brain malignancy in adults, with a median overall survival of 12–15 months despite maximal therapy. Resistance to the standard chemotherapeutic agent temozolomide (TMZ) remains a near-universal clinical obstacle, motivating the development of mechanistically distinct therapeutic strategies. ORAI1-mediated store-operated calcium entry (SOCE) is aberrantly elevated in GBM and drives pro-survival AKT signaling, including MDM2-dependen...

Understanding Glyphosate-Paraquat and Glufosinate-Paraquat Synergistic Herbicidal Effect in Plants See

The global incidence of herbicide resistance has reached an alarming rate, similar to that observed for antibiotic resistance. This research aims to investigate the emerging synergistic effects of three widely used herbicides: Glufosinate, Paraquat, and Glyphosate. The combinations of these herbicides have demonstrated promising synergistic effects, which could provide a feasible solution to herbicide resistance arising from unregulated reliance on a single mode of action. According to p...

Molecular Identification of Culturable Endophytic Bacterial Species from Leaves of Piper crocatum See

Antimicrobial resistance (AMR) poses a severe threat to global public health, driving an urgent need for novel therapeutic agents to replace ineffective conventional antibiotics. Endophytic microorganisms, which reside within plant tissues and can synthesize diverse bioactive secondary metabolites, offer a potential source for various novel antimicrobials. In this study, Endophytic Bacterial Species were investigated from the leaves of Piper crocatum, which is a medicinal plant widely used in...

Evaluation of Antibiofilm Activity and Safety of Binahong Plant Extracts Against Foodborne Bacteria for Potential Food-Contact Applications See

Biofilm-forming bacteria, including Staphylococcus aureus and Salmonella typhimurium, can persist on food-contact surfaces, increasing resistance to cleaning, disinfection, and antimicrobial agents, highlighting the need for alternative antimicrobial agents. This study evaluated the antibacterial, antibiofilm, and cytotoxic effects of aqueous Binahong (Anredera cordifolia) extract. The cytotoxicity was evaluated using the MTT assay on HaCaT cells, antibacterial activity was determined usin...

In Vitro Characterization Of Lgr6-Derived Cell Response To Differential Regimens In ESCC Organoids See

Esophageal squamous cell carcinoma (ESCC) is one of the most prevalent and aggressive forms of esophageal cancer, with poor survival outcomes largely due to late-stage diagnosis and therapeutic resistance. Current treatment for advanced ESCC commonly involves neoadjuvant chemotherapy using the docetaxel, cisplatin, and 5-fluorouracil (DCF) regimen; however, relapse and chemoresistance remain major clinical challenges. Increasing evidence suggests that distinct cell populations with stem/proge...