Please use this identifier to cite or link to this item: http://repository.i3l.ac.id/jspui/handle/123456789/840
Full metadata record
DC FieldValueLanguage
dc.contributor.authorNovella, Wenny-
dc.date.accessioned2023-11-15T07:50:50Z-
dc.date.available2023-11-15T07:50:50Z-
dc.date.issued2023-06-12-
dc.identifier.urihttp://repository.i3l.ac.id/jspui/handle/123456789/840-
dc.description.abstractPediococcus acidilactici is a non-pathogenic probiotic bacteria that can improve gut health by adhering to intestinal cells and inhibiting pathogen adherence. To create an easy-to-use product, probiotics are often produced in powdered form using spray drying. However, this drying method can affect the probiotic's viability and functionality. This study examined the impact of spray drying inlet temperature on P. acidilactici's ability to adhere and inhibit pathogen adherence. The hydrophobicity of the cells was measured before and after spray drying, found that spray-dried P. acidilactici maintained high hydrophobicity (96.58-98.92%). The study also evaluated the adherence rate of P. acidilactici to HT-29 cells before and after spray drying, showing a decrease in adherence rate by 37.68%, 30.38%, and 32.64% (SD120, SD150, SD170) after spray drying. Additionally, P. acidilactici's ability to inhibit pathogen adherence, specifically Listeria monocytogenes and Staphylococcus aureus, was assessed by counting pathogen attachment to HT-29 cells in the presence of P. acidilactici. Without P. acidilactici, S. aureus exhibited a low adherence rate (1.19 ± 0.008%), while L. monocytogenes adherence rate was 14.34 ± 0.033%. The presence of free-cell P. acidilactici significantly reduced L. monocytogenes adherence to 88.16 ± 0.07%. However, after spray drying, P. acidilactici increased L. monocytogenes adherence (ranging from 46.19% to 10.47%) possibly due to the presence of wall material like gum arabic and whey protein isolate. The inlet temperature during the spray drying process did not affect the cell surface hydrophobicity of the P. acidilactici, significantly impacting adherence and pathogen inhibition. Further research is needed to identify the effect of wall material on adhesion and inhibit pathogen adherence.en_US
dc.language.isoenen_US
dc.publisherIndonesia International Institute for Life Sciencesen_US
dc.relation.ispartofseriesBT 23-024;T202306024-
dc.subjectediococcus acidilacticien_US
dc.subjectspray dryingen_US
dc.subjectadherenceen_US
dc.subjectadherence inhibitionen_US
dc.subjectcell surface hydrophobicityen_US
dc.titleEffect of Spray-drying Towards Pediococcus acidilactici Adherence Ability to Reduce Pathogen Adhesion to The Surface of Intestinal Epithelial Cellsen_US
dc.typeThesisen_US
Appears in Collections:Biotechnology

Files in This Item:
File Description SizeFormat 
BT 23-024_Wenny Novella.pdf
  Restricted Access
Full Text942.68 kBAdobe PDFView/Open Request a copy
Abstract.pdfAbstract67.6 kBAdobe PDFView/Open
Chapter 1.pdfChapter 186.66 kBAdobe PDFView/Open
Cover.pdfCover75.98 kBAdobe PDFView/Open
References.pdfReferences159.37 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.