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http://repository.i3l.ac.id/jspui/handle/123456789/805| Title: | In Vitro Testing Of Deodorants’ Antibacterial Activity Against Bacteria Isolated From Human Axilla |
| Authors: | Widjojo, Clara Ayu Inaray, Ivetta Izhora Hartrianti, Pietradewi Devanthi, Putu Virgina Partha Pramanda, Ihsan Tria |
| Issue Date: | 7-Sep-2022 |
| Publisher: | Direktorat Jendral Kekayaan Intelektual |
| Series/Report no.: | EC00202262208;000377942 |
| Description: | The research protocol aims to test and compare the antimicrobial activity of different kinds of deodorant by adopting in vitro testing methods against Indonesian males’ axillary microbiome. Popular antimicrobial assays such as spot-plating assay and time-kill assay can be adapted to mimic clinical trials on humans in treating bacteria with deodorant on agar or in test tubes/well plates. Spot-plating assay tests the deodorant’s antimicrobial performance through bacterial zone of inhibitions, whereas, time-kill assay measures bacterial reduction over time for samples treated with deodorant. The deodorants are tested against four dominant bacteria isolated from Indonesian male subjects’ axilla: Corynebacterium tuberculostearicum, Micrococcus luteus, Staphylococcus hominis and Staphylococcus epidermidis. Before undergoing these tests, bacterial growth curve and standard curve need to be generated to ensure consistency and optimacy throughout the study. Additionally, preliminary tests to determine the minimum inhibitory concentration of positive control (antibiotic) and bacteria-to-deodorant ratio are required. |
| URI: | http://repository.i3l.ac.id/jspui/handle/123456789/805 |
| Appears in Collections: | Pharmacy |
Files in This Item:
| File | Description | Size | Format | |
|---|---|---|---|---|
| 6. Sertifikat_EC00202262208_In Vitro.pdf | Certificate | 2.05 MB | Adobe PDF | View/Open |
| 5. In Vitro Protocol - Skinproof.pdf | Module | 1.37 MB | Adobe PDF | View/Open |
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