The Effects of Spray Drying Inlet Air Temperature on Bile Salt Hydrolase Activity of Pediococcus acidilactici for Cholesterol Lowering Property

Abstract

ile salt hydrolase (BSH) is important to help bile salt deconjugation, converting bile salt into a more hydrophobic form which helps to alleviate cholesterol level in the body. BSH is a N-terminal nucleophile superfamily enzyme, and frequently found in lactic acid bacteria (LAB). Spray drying, as one of preservation methods, is often applied for commercial production, and known to reduce the viability of probiotics. Although there is a rising recognition of lactic acid bacteria, especially Pediococcus acidilactici, as potential probiotics, less studies have been conducted to investigate the effect of spray drying temperatures to BSH activity in P. acidilactici. Thus, this study aimed to analyze the impact caused by different inlet air temperatures of spray drying towards BSH activity of P. acidilactici. BSH activity derived from spray dried P. acidilactici samples produced from three different inlet temperatures (120 o C, 150 o C, and 170 o C) were investigated qualitatively and quantitatively. Qualitative measurement was using a living cell, where all of the samples produced similar bile salt deconjugation activity without any significant differences (p > 0.05). On the other hand, quantitative measurement was only able to use the samples supernatant, because the extraction methods were not effective. Quantitative data which showed low specific activity were disputable because the wall material (WM) of the spray dried samples was also detected in the colorimetric assays and resulted in overestimation of concentration and activity. Despite the issue, this study showed promising activity of BSH from P. acidilactici after spray drying in all inlet temperatures.