In Vitro Study of Marchantia Paleacea Potential as Cytoprotective Agents on Hacat Cell Lines Under Oxidative Stress

Abstract

In this study, the potential of Marchantia paleacea, a bryophyte that grows in the Cibodas Botanical Garden, was studied for its cytoprotective capability against oxidative stress. H was used. Although bryophytes have been used in traditional medicine for centuries, this group of plants is still poorly studied. Bryophytes have been shown to contain metabolites such as flavonoids, polyphenols, alkaloids, and saponins that can be used as anti-aging agents due to their anti- inflammatory effects. It was hypothesized that M. paleacea may have anti-aging properties that can be used as a cytoprotective agent against oxidative stress. To test the cytoprotective effect of M. paleacea, MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H- tetrazolium) assay was used. To induce the oxidative stress, hydrogen peroxide (H) was used in this study. The results show that M. paleacea extracts may have cytoprotective effects against Hat 22 ppm. However, further study and validation was still needed to validate the biological significance of the results.