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Title: | Single-cell Analysis of Long Non-coding RNAs (lncRNAs) in Tumor-Associated Macrophages (TAMs) in the Glioblastoma Microenvironment |
Authors: | Giannetta Surya, Angela Merici |
Keywords: | Glioblastoma, Single cell RNA Sequencing, Tumor Associated Macrophages, long non-coding RNAs, PCED1B-AS1 PCED1B-AS1 long non-coding RNAs Tumor Associated Macrophages Single cell RNA Sequencing |
Issue Date: | 31-Aug-2024 |
Publisher: | Indonesia International Institute for Life-Sciences |
Series/Report no.: | BI 24-007;T202409014 |
Abstract: | Glioblastoma (GBM) is the most severe type of brain tumor with poor prognosis and low survival rate, characterized by molecular heterogeneity and immunosuppressive microenvironment which pose a significant challenge in improving treatment strategies. It is found that the abundance of tumor- associated macrophages (TAMs) in the GBM microenvironment contributes significantly to tumor progression, yet the understanding of the underlying mechanism remains limited. Long non-coding RNAs (lncRNAs) have emerged as key regulators in cancer biology, including their involvement in tumor progression mechanisms mediated by TAMs. In this research, we performed single-cell RNA sequencing analysis on 17,803 cells from 6 glioma patient samples to profile the GBM tumor microenvironment landscape. We identified five TAM subtypes distinguished by unique gene expression profiles, and highlighted the two primary TAM ontogenies: brain-resident microglia and infiltrating monocyte-derived macrophages. Our analysis revealed PCED1B antisense RNA 1 (PCED1B- AS1) as a significantly expressed lncRNA in TAMs, particularly associated with immune-related pathways. The enrichment of PCED1B-AS1 showed a context-dependent impact on prognosis across different TAM clusters, suggesting its role in regulating immune cell functions. We demonstrated that PCED1B-AS1 potentially plays a crucial role in GBM microenvironment through its interaction with TAMs. Our findings suggest that targeting PCED1B-AS1 could offer a novel therapeutic strategy for GBM. The use of scRNA-seq was pivotal in uncovering the heterogeneity of TAMs and the specific roles of lncRNAs within the tumor microenvironment. |
URI: | http://repository.i3l.ac.id/jspui/handle/123456789/1281 |
Appears in Collections: | Bioinformatics |
Files in This Item:
File | Description | Size | Format | |
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Angela Merici Giannetta Surya.pdf Restricted Access | 3.66 MB | Adobe PDF | View/Open Request a copy |
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