Please use this identifier to cite or link to this item:
Title: A-to-I RNA Editing in Cardiomyocytes: Profiling the Landscape in Cell Differentiation and Ischemic Heart Disease
Authors: Fachrul, Muhamad
Keywords: RNA editing
Cardiovascular diseases
Issue Date: 28-Aug-2018
Publisher: Indonesia International Institute for Life Sciences
Series/Report no.: BI 18-001;T201809021
Abstract: The unceasing progress in sequencing technologies and analytical methods in recent years has resulted in the rise in attention to epitranscriptomics – a form of which is RNA editing, the most common form being A-to-I RNA editing. ADAR-mediated A-to-I RNA editing is well-studied in cancers but is relatively uncharted in the human heart. In this study, two datasets were analyzed for A-to-I RNA editing activity, representing cardiac differentiation and ischemic heart disease (IHD). Identification of editing sites was done through a pipeline mainly utilizing GATK’s variant calling suite as the core. A total of 35,948 and 24,797 unique editing sites were identified in the differentiation and IHD datasets, respectively, mainly affecting Alu repeats located in 3’UTR and intronic regions. LIN28A was significantly differently edited across the cardiac differentiation time course, though this might not be exclusive to cardiomyocytes. In IHD, RYR2 and FKBP5 were among the genes corresponding to the top editing sites. A-to-I RNA editing activity was also found to induce protein recoding in both datasets, corresponding to transcripts known to be edited in cancers such as SRP9, COG3, KANSL1, and COPA. The exact functional implications remain to be determined, though this study provides a baseline of the landscape of A-to-I RNA editing in cardiomyocyte differentiation and disease state.
Appears in Collections:Bioinformatics

Files in This Item:
File Description SizeFormat 
T201809021_BI_Muhamad Fachrul_14010031.pdf
  Restricted Access
Full Text2.52 MBAdobe PDFView/Open Request a copy
cover.pdfCover2.48 MBAdobe PDFView/Open
Chapter 1.pdfChapter 12.48 MBAdobe PDFView/Open
Abstract.pdfAbstract2.48 MBAdobe PDFView/Open
References.pdfReferences2.48 MBAdobe PDFView/Open

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.