In Vitro Methods of Antimicrobial Activity Measurement of Erha’s Deodorant on Staphylococcus Hominis and Micrococcus Luteus

Abstract

Human body odor has been a long-term problem for most people, which affects the physiological condition of the sufferers due to the social stigma given by society. It is caused by the mixtures of sweats and skin microbiota located in the axillary area. Staphylococcus hominis and Micrococcus luteus are some of the skin microbiota found in human skin, and these bacteria are responsible for producing the pungent component of human body odor. Because of that, deodorant was produced to control the skin microbiota, as well as mask the body odor. Erha, one of Indonesia’s dermatology companies has formulated a natural deodorant using horsetail extract and sage oil as the main ingredient. The antimicrobial testing was done by 2 in vitro methods; Spot Plating Assay and Time-Kill assay, and a growth curve is also constructed. Aside from deodorant from Erha, deodorant from Rexona and The Bath Box are also used for comparison. The result of Erha’s deodorant on Spot Plating Assay is the most significant among others due to the high average of ZOI, while in Time Kill Assay, Rexona’s deodorant shows the most significant efficiency due to a 100% bacterial reduction percentage. To improve this study, adding more data using higher OD and constructing a standard curve would be beneficial to make the data more accurate and credible.