http://http://repository.i3l.ac.id:80/jspui/handle/123456789/368 EP Report for Food Science and Nutrition Student 2026-01-08T11:31:00Z http://http://repository.i3l.ac.id:80/jspui/handle/123456789/1214 Title: Molecular Detection of Mannonate Dehydratase Genes and its Transcriptional Activity in Compost-Derived Microbial Samples Authors: Kurniadi, Anya Callista Abstract: Bioplastics such as PLA (Polylactic acid) have been promoted and utilized as a sustainable alternative to synthetic plastics which is responsible for the global plastic waste problem. However, PLA has a limitation where its biodegradability is limited to high temperature conditions which are not provided in the current system for managing PLA waste. A recent development in a PLA-degrading compost system led by Associate Professor Shu Yuan Yang’s lab that could break down the material without the need for such extreme temperatures could be key to enhancing PLA’s sustainability. This study investigates the presence and transcriptional activity of mannonate dehydratase genes that have previously been identified from metagenomics analysis in microbial communities from the trained compost samples. Nested PCR and cDNA synthesis was used to amplify targeted gene regions and confirm the transcriptional activity. While positive results in regards to the detected genes sequences were obtained for all 3 reference sequences, and cDNA in the 2A18 primer assays, the gene’s functional potential and expression at a protein level remains unverified. The findings in this study provides a foundation in understanding the molecular mechanisms of PLA degradation within the trained compost system to aid future optimization and functional characterization. 2025-01-31T00:00:00Z http://http://repository.i3l.ac.id:80/jspui/handle/123456789/1213 Title: Bioenergy Harvesting from Human Sweat Using a Photocatalytic Polypyrrole/MoS2 Composite Electrode Authors: Susanto, Jennifer Marsha Abstract: As the demand for personal wearable devices escalates, lithium waste has become an environmental issue, impulsing the necessity to uncover potential renewable energy sources. In that regard, the study proposed an electropolymerized pyrrole that could conceivably act as the conductive support, empowering the electrochemical reaction to generate electricity within the proposed biofuel-powered system, together with MoS2 with its electronic properties and high photogenerated electron density, synthesizing a composite-based electrode to convert human sweat ions: potassium, sodium, calcium, and chloride, etc. to be bioenergy. From the study, it was found that by using 0.084 M of pyrrole with a 3600-s chronoamperometry, the current generated by the PPy/MoS2 composite was optimum as evaluated with visible light as well as having a minimum impedance compared to the others. Through the samples, the electropolymerization yielded proof of the composite existence by demonstrationing MoS2 as a large rigid structure, with PPy anchored towards it along with the peak combination between the consituent atoms in FTIR, XRD, and Raman as well as XPS to locate signals of the element orbitals along with the atomic percentage of the composite and its constituent separately. The too-low current made the LED highly dim to no light during the final demonstration; hence, more studies regarding the factor of incident light angle towards the catalyst were needed. 2025-01-31T00:00:00Z http://http://repository.i3l.ac.id:80/jspui/handle/123456789/1212 Title: Internship at PT MULTI BINTANG INDONESIA TBK Quality Department Authors: Nathania, Angeline Abstract: PT Multi BINTANG Indonesia is a multi-beverage company as part of the HEINEKEN group with over 90 years of history. The quality of beer produced here is of the utmost importance and at the heart of it is the Quality Department. In the Main Lab of the Tangerang Brewery where the author was assigned, responsibilities were assigned ranged from physical analysis, entrance control, to sensory analysis. All of these analyses serve to maintain the quality of every beer that is made in the facility starting from the raw materials, different stages in the beer making such as Wort, and final products. These tasks helped the author grow not only in the soft skills but also in hard skills. 2025-01-31T00:00:00Z http://http://repository.i3l.ac.id:80/jspui/handle/123456789/1211 Title: Sweet Product Application Internship at PT Indesso Niagatama Authors: Rahma, Adinda Aulia Abstract: This internship report records the six-month of professional work experience at PT Indesso, focusing on the product innovation and development at Sweet Innovation and Application Department. A lot of projects were conducted throughout the six-month period, including creating applications for cookies, chocolate, gummy, and other product requests. The project that was detailedly explained was reformulating a chocolate-flavored cream filling by reducing cocoa powder content by 50% while maintaining the sensory properties including flavor, texture, and color comparable to the benchmark product. The reformulation process included tons of trials in flavorants, colorant, and alternative ingredients such as cocoa shell-based products to enhance texture and mouthfeel. The author's responsibilities included assisting with flavor and color formulation, preparing base products, conducting sensory evaluations, and supporting cost-reduction strategies for clients projects. Additional tasks such as determining price strategies for natural colorants and supporting sensory tests such as triangle tests were also done by the author. The internship provided hand-on experience to industry practices, enhanced knowledge in product development, and developed soft skills as teamwork, problem solving, collaboration in addressing client requirements while adapting to market current trends, such as rising raw material costs. 2025-01-31T00:00:00Z