http://http://repository.i3l.ac.id:80/handle/123456789/59 Student Final Project, Thesis, and Dissertation produced by Students of Indonesian International Institute for Life Sciences. 2026-01-15T09:26:02Z http://http://repository.i3l.ac.id:80/handle/123456789/1334 Title: An Evaluation of Physiology for Plant Growth Promoting Microorganisms from Takakura Compost Isolates Authors: Raynard, Nathanael Abstract: Degradation of soil health through excessive usage of chemical fertilizer has led to decreased agricultural production, to mitigate the effects of soil degradation, compost containing PGPM should be utilized. This study focuses on characterizing Plant-Growth Promoting Microorganisms in Takakura compost isolates, by identifying nitrogen-fixing, and phosphate solubilization capabilities. PGPMs have an important role in the growth and health of plants, by increasing the availability of nutrients, synthesizing growth hormones, increasing resistance to abiotic stresses through the production of stress hormones, and protecting against pathogenic microbes. The data gained from the PGP experiment using isolates from Takakura compost shows that 36 isolates can survive in nitrogen-free medium while 4 isolates have exhibited phosphate solubilization capabilities, which could be concluded that the isolates have PGP capabilities and can be used as soil inoculants. 2025-08-31T00:00:00Z http://http://repository.i3l.ac.id:80/handle/123456789/1333 Title: Optimization of Salt and Sulfur Sources for Bacillus Bacteria Fermentation in Keratin Extraction from Chicken Feathers Authors: Cahyadi, Ignatius Khaisa Abstract: Chicken feathers contain roughly around 90% protein in the form of keratin, which has a lot of uses in the production of goods such as animal feed, biofilm, fertilizer, and wood adhesive. Common disposal methods like burning require energy and emit carbon dioxide, while dumping and burying may result in the spread of pathogens. On the other hand, currently available extraction methods such as hydrolysis, dissolution in ionic liquids, microwave technique, steam explosion technique, and thermal hydrolysis have numerous downsides. Thus, the aim of this research was to explore a more environmentally friendly and efficient method to extract keratin by using bacteria. The first step was to screen for the best bacterial strain from the genus Bacillus that can degrade chicken feathers to extract keratin through the use of keratinase enzyme. Afterwards, nutrients for the strain were optimized in an attempt to increase keratin yield and quality. After screening, the Bacillus subtilis strain N10ND was chosen for nutrient optimization using supplementary NaCl (sodium chloride) and MgSO4 (magnesium sulfate). It was found that the addition of NaCl and MgSO4 had no significant impact on the quantity and quality of the keratin yield. 2025-08-31T00:00:00Z http://http://repository.i3l.ac.id:80/handle/123456789/1332 Title: A Semi-Quantitative Investigation on the Effect of Methyl Jasmonate Treatment in Saga Culture to Enhance High Value Triterpenoids Saponin Authors: Prabawa, Matthew Ilyas Satria Abstract: The medicinal vine Abrus precatorius, sometimes referred to as Saga, is indigenous to tropical Asia and India. Because of its pharmacological qualities, it has a long history in traditional medicine. According to a recent study, Saga may have medicinal uses in the area of respiratory health, particularly given its effectiveness in treating diseases like COVID-19. Despite its pharmaceutical prospects, the biosynthesis regulation of its active compound remains limited, especially with regard to its notable triterpenoid saponin called abrusosides. In order to address this gap, the present study investigates the role of methyl jasmonate in increasing the accumulation of triterpenoid compounds. Methyl jasmonate is known to induce secondary metabolite production, such as triterpenoids, as part of the plant defense response. Inspired by several studies that reported an increase in triterpenoid accumulation post methyl jasmonate treatment, this study aims to test the effect of methyl jasmonate treatment at various concentrations on the production of triterpenoid saponins in A. precatorius. This study evaluated the effect of methyl jasmonate on triterpenoid saponin accumulation in Abrus precatorius leaves and roots. The highest saponin levels were observed at 0.1 mM methyl jasmonate, while higher concentrations (0.3 mM and 0.5 mM) led to a decline.These results suggest that low-dose methyl jasmonate optimally induces saponin biosynthesis, whereas higher doses may suppress it due to stress-related feedback. 2025-08-31T00:00:00Z http://http://repository.i3l.ac.id:80/handle/123456789/1331 Title: Identification of ROS Inhibition Activity in Pediococcus acidilactici TAT-1 through Whole Genome Sequencing and Antioxidant Bioassays Authors: Chrisanty, Fransisca Arlynn Abstract: Pediococcus acidilactici is a facultative anaerobic bacterium that prefers microaerophilic environments with low oxygen. This study aimed to evaluate the ROS inhibition activity of P. acidilactici strain TAT1 through Whole Genome Sequencing data and antioxidant bioassays, such as the DPPH assay, superoxide scavenging activity assays, and catalase activity assay. WGS analysis revealed the presence of multiple antioxidant-related genes, though superoxide dismutase (SOD) genes were absent. Despite this, the DPPH assay showed strong antioxidant activity at higher concentrations of the cell-free supernatant (CFS), and the superoxide radical scavenging assay confirmed superoxide scavenging even in the absence of SOD. Additionally, hydrogen peroxide resistance was observed in the catalase activity assay, suggesting possible catalase activity, though the result remains inconclusive due to methodological limitations. Understanding the antioxidant capacity of P. acidilactici TAT1 is crucial for exploring its potential applications in probiotic development and industrial fermentation, where oxidative stress tolerance is a key factor for bacterial viability and functionality. 2025-08-31T00:00:00Z