Final Paper of Pharmacy Student http://http://repository.i3l.ac.id:80/handle/123456789/90 2026-04-21T18:37:19Z 2026-04-21T18:37:19Z Tio, Gregory Adriel http://http://repository.i3l.ac.id:80/handle/123456789/1391 2026-01-22T08:29:04Z 2025-08-31T00:00:00Z

Title: Formulation and Evaluation of Wound Healing Properties of Camellia Sinensis Extract and Allantoin Based Anti-Acne Serum Authors: Tio, Gregory Adriel Abstract: Skin health is a paramount concern, which affects individuals of all ages, impacting not only physical health but also psychological well-being. Acne vulgaris is a chronic skin condition characterized by diverse lesions, such as open and closed comedones, pustules, papules, and cysts, which vary with the stage of progression. Inadequate treatment or severe cases can result in atrophic scar formation. Atrophic scars can be challenging to remove completely, and the degree of success depends on various factors. Green tea, specifically known as Camellia sinensis, offers an abundant quantity of beneficial properties, one of them being its polyphenolic components, particularly catechins. These compounds enhance collagen synthesis, which is essentially the early stages of wound healing. Allantoin is widely used in cosmetics and pharmaceuticals for its therapeutic benefits and it helps the transition from wound inflammation to proliferation. It offers antioxidant, anti-inflammatory, antimicrobial properties, and facilitates the shedding of dead skin cells, aiding wound healing. The evaluation of the experiment will be done through scratch assays in which it cultivates a single layer of cells in a multiwell assay plate until it reaches full coverage, generating a deliberate 'wound' or gap within this cell layer where cells are able to migrate, and then overseeing the process of cells repopulating the scratched area to measure and assess cell migration. Thus, this research aims to formulate and evaluate a skincare solution that combines the wound-healing properties of green tea extract with the known benefits of allantoin in an acne scar serum.

2025-08-31T00:00:00Z Putri, Stevanie http://http://repository.i3l.ac.id:80/handle/123456789/1390 2026-01-22T08:08:02Z 2025-08-31T00:00:00Z

Title: In Vitro Analysis of Antibacterial Efficacy of Deodorant Formulations against Staphylococcus aureus and Sensory Evaluation Authors: Putri, Stevanie Abstract: Body (axillary) malodor is a common problem that adversely affects social interactions due to the interaction between sweat glands and bacterial activity such as Staphylococcus aureus. To address this, three deodorant formulations (F1, F2, and F3) each containing active ingredients of 0.5%, 0.3%, and 0.3% concentration with an additional boosting agent were being evaluated through in vitro testing, including bacterial growth and standard curve, Kirby-Bauer test, and time-kill assay. All formulations exhibit antibacterial activity, however to further confirm the potency of antibacterial ingredients, a preliminary test should be conducted prior performing time-kill assay. No inhibition zones were observed however time kill assay demonstrated a complete bacterial eradication. Additionally, consumer acceptability was tested through sensory evaluation using 5-point hedonic scale from "dislike extremely" to "like extremely" and 5-point QDA scale from 1-non detectable attribute to 5-clearly detected attribute. Sensory attributes including odor, transparency, color, stickiness, spreadability, odor intensity, drying speed, amount of residue, stinging sensation, and overall likeness were assessed. No significant differences were found between F1, F2, and F3, signifying that differences of active ingredient concentration and an additional boosting agent did not change any sensory perception. More distinct differentiation might be observed under higher concentration differences and longer evaluation time. This study aims to determine optimal balance between functional efficacy and consumer appeal by assessing antibacterial activity and sensory attributes of different deodorant formulations.

2025-08-31T00:00:00Z Cahya, Rachel Josephine http://http://repository.i3l.ac.id:80/handle/123456789/1389 2026-01-22T07:55:35Z 2025-08-31T00:00:00Z

Title: Analytical Method Development of Water Soluble Multivitamins Utilizing LC-MS/MS Authors: Cahya, Rachel Josephine Abstract: Accurate analysis of multivitamin formulations is necessary to ensure product quality, efficacy, and safety. This research aimed to develop and validate an analytical method for the simultaneous identification and quantification of water-soluble vitamins using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Method development involved system suitability testing, linearity, accuracy, precision, and robustness evaluation. The LC-MS/MS method enabled the successful detection of multiple vitamins with optimized chromatographic and mass spectrometric parameters. The developed method showed good linearity for most vitamins, including thiamine, riboflavin, niacin, niacinamide, pantothenic acid, pyridoxine HCl. Recovery and precision showed significant deviations due to instrumental limitations and sample preparation variability. Vitamin C was excluded due to its instability under analytical conditions. Despite limitations, this method demonstrates potential for routine multivitamin screening, especially in pharmaceutical quality control. Further optimization, such as improved weighing accuracy and the use of specialized chromatographic columns could enhance performance and broaden its applicability.

2025-08-31T00:00:00Z Loavenia, Michelle http://http://repository.i3l.ac.id:80/handle/123456789/1388 2026-01-22T07:46:53Z 2025-08-31T00:00:00Z

Title: In Vitro Evaluation of Sacha Inchi Ferment Filtrate as a Protective Agent Against UVB in HaCaT Cells Authors: Loavenia, Michelle Abstract: Human skin is a primary barrier against environmental pollutants and infectious agents, including UV radiation, which contributes to photoaging. Sacha inchi, a plant with bioactive compounds like phenolic and flavonoids, has potential as a photoprotective agent against UVB radiation. However, studies have not investigated its potential as an alternative material source for protecting skin from UVB-induced damage. This research aims to evaluate the cytoprotective effects of Sacha inchi ferment filtrate against UVB-induced damage in human keratinocytes (HaCaT cells). Characterization of Sacha inchi ferment filtrate including total protein concentrations, total phenolic content, total flavonoid content, and FRAP assay was done. The cytotoxicity and cytoprotective evaluation was done with MTS assays to quantify the cell viability. The protein concentrations of fermented Sacha inchi showed significant increase while both total phenolic and flavonoid content did not exhibit significant differences compared to the Sacha inchi milk. Sacha inchi samples that contained sediment showed higher cytotoxicity due to the presence of bioactive compounds in the sediment. Higher cytoprotective ability was also shown in non-fermented Sacha inchi milk with sediment. Despite higher protein content, both fermented Sacha inchi samples did not show a higher cytoprotective effect than non-fermented Sacha inchi milk. Further studies on Sacha inchi ferment filtrate should be done such as compound characterization, comet assay, and gene expression to provide more information on the bioactive compounds contained in the ferment filtrate and pathway that causes cytoprotective effect

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