Undergraduate Thesis on Faculty of Life Sciences http://http://repository.i3l.ac.id:80/handle/123456789/80 2026-04-21T16:57:57Z 2026-04-21T16:57:57Z Lee, Virginia Gladys http://http://repository.i3l.ac.id:80/handle/123456789/1413 2026-02-11T04:21:32Z 2023-08-31T00:00:00Z

Title: Prediction of T-Cell and B-Cell Epitopes from Protein Allergen Tropomyosin That Are Presented by Major Hla Alleles in The Indonesian Population Authors: Lee, Virginia Gladys Abstract: Shellfish allergy is one of the highest prevalence allergies in the world, accounting for 7.7% of the population including Indonesia. Thus, the development of T cell epitope and B cell epitope based vaccines play an important role in future prospects for alternative treatment for allergy. The vaccine aims to induce the immune system so it will not react towards the allergen though the body already went through sensitization. In this experiment, immunoinformatics methods were used to predict the T cell and B cell epitopes from Pen a 1 allergen, tropomyosin from Penaeus aztecus. T cell epitopes were obtained from NetCTLpan for HLA Class I binding peptide and NetMHCIIpan for HLA Class II binding peptide. As for B cell epitopes, three different servers that are complementary to each other were used; BepiPred 2.0, IEDB B Cell Epitope Prediction Tool, and ABCpred. T cell epitopes were analyzed further for their immunogenicity, IFN-gamma-inducing ability, cross-reactivity against human peptides, and conservancy in other tropomyosin sequences. The vaccine was then constructed using promiscuous T cell and B cell epitopes using beta-defensin as adjuvant and appropriate linkers. The vaccine construct has good population coverage and therefore potential to be developed as an allergen immunotherapy for the Indonesian population.

2023-08-31T00:00:00Z Tio, Gregory Adriel http://http://repository.i3l.ac.id:80/handle/123456789/1391 2026-01-22T08:29:04Z 2025-08-31T00:00:00Z

Title: Formulation and Evaluation of Wound Healing Properties of Camellia Sinensis Extract and Allantoin Based Anti-Acne Serum Authors: Tio, Gregory Adriel Abstract: Skin health is a paramount concern, which affects individuals of all ages, impacting not only physical health but also psychological well-being. Acne vulgaris is a chronic skin condition characterized by diverse lesions, such as open and closed comedones, pustules, papules, and cysts, which vary with the stage of progression. Inadequate treatment or severe cases can result in atrophic scar formation. Atrophic scars can be challenging to remove completely, and the degree of success depends on various factors. Green tea, specifically known as Camellia sinensis, offers an abundant quantity of beneficial properties, one of them being its polyphenolic components, particularly catechins. These compounds enhance collagen synthesis, which is essentially the early stages of wound healing. Allantoin is widely used in cosmetics and pharmaceuticals for its therapeutic benefits and it helps the transition from wound inflammation to proliferation. It offers antioxidant, anti-inflammatory, antimicrobial properties, and facilitates the shedding of dead skin cells, aiding wound healing. The evaluation of the experiment will be done through scratch assays in which it cultivates a single layer of cells in a multiwell assay plate until it reaches full coverage, generating a deliberate 'wound' or gap within this cell layer where cells are able to migrate, and then overseeing the process of cells repopulating the scratched area to measure and assess cell migration. Thus, this research aims to formulate and evaluate a skincare solution that combines the wound-healing properties of green tea extract with the known benefits of allantoin in an acne scar serum.

2025-08-31T00:00:00Z Putri, Stevanie http://http://repository.i3l.ac.id:80/handle/123456789/1390 2026-01-22T08:08:02Z 2025-08-31T00:00:00Z

Title: In Vitro Analysis of Antibacterial Efficacy of Deodorant Formulations against Staphylococcus aureus and Sensory Evaluation Authors: Putri, Stevanie Abstract: Body (axillary) malodor is a common problem that adversely affects social interactions due to the interaction between sweat glands and bacterial activity such as Staphylococcus aureus. To address this, three deodorant formulations (F1, F2, and F3) each containing active ingredients of 0.5%, 0.3%, and 0.3% concentration with an additional boosting agent were being evaluated through in vitro testing, including bacterial growth and standard curve, Kirby-Bauer test, and time-kill assay. All formulations exhibit antibacterial activity, however to further confirm the potency of antibacterial ingredients, a preliminary test should be conducted prior performing time-kill assay. No inhibition zones were observed however time kill assay demonstrated a complete bacterial eradication. Additionally, consumer acceptability was tested through sensory evaluation using 5-point hedonic scale from "dislike extremely" to "like extremely" and 5-point QDA scale from 1-non detectable attribute to 5-clearly detected attribute. Sensory attributes including odor, transparency, color, stickiness, spreadability, odor intensity, drying speed, amount of residue, stinging sensation, and overall likeness were assessed. No significant differences were found between F1, F2, and F3, signifying that differences of active ingredient concentration and an additional boosting agent did not change any sensory perception. More distinct differentiation might be observed under higher concentration differences and longer evaluation time. This study aims to determine optimal balance between functional efficacy and consumer appeal by assessing antibacterial activity and sensory attributes of different deodorant formulations.

2025-08-31T00:00:00Z Cahya, Rachel Josephine http://http://repository.i3l.ac.id:80/handle/123456789/1389 2026-01-22T07:55:35Z 2025-08-31T00:00:00Z

Title: Analytical Method Development of Water Soluble Multivitamins Utilizing LC-MS/MS Authors: Cahya, Rachel Josephine Abstract: Accurate analysis of multivitamin formulations is necessary to ensure product quality, efficacy, and safety. This research aimed to develop and validate an analytical method for the simultaneous identification and quantification of water-soluble vitamins using Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS). Method development involved system suitability testing, linearity, accuracy, precision, and robustness evaluation. The LC-MS/MS method enabled the successful detection of multiple vitamins with optimized chromatographic and mass spectrometric parameters. The developed method showed good linearity for most vitamins, including thiamine, riboflavin, niacin, niacinamide, pantothenic acid, pyridoxine HCl. Recovery and precision showed significant deviations due to instrumental limitations and sample preparation variability. Vitamin C was excluded due to its instability under analytical conditions. Despite limitations, this method demonstrates potential for routine multivitamin screening, especially in pharmaceutical quality control. Further optimization, such as improved weighing accuracy and the use of specialized chromatographic columns could enhance performance and broaden its applicability.

2025-08-31T00:00:00Z